H3T3pK4ac | Histone H3 (ac Lys4, p Thr3)
AS16 3170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

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Product Information
Immunogen
KLH-conjugated synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Quantity
50 ĩg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Caenorhabditis elegans, Human
Predicted reactivity
Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
Dot Blot using anti-H3T3pK4ac antibodies. Lane 1: T3p. Lane 2: T3pK4me1. Lane 3: T3pK4me2. Lane 4: T3pK4ac. Lane 5: T3pR2me2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

Immunofluorescence using anti-H3T3pK4ac antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4ac is nuclear and chromosomal. Staining: H3T3pK4ac is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight® 550 (red).

Western Blot using anti-phospho-acetyl-Histone H3 antibodies (H3T3pK4ac). Lane 1: HeLa histone extracts. Lane 2. NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 00 for 45 min at RT.

Dot Blot using anti-H3T3pK4ac antibodies. Lane 1: T3p. Lane 2: T3pK4me1. Lane 3: T3pK4me2. Lane 4: T3pK4ac. Lane 5: T3pR2me2s. Lane 6: K4 unmodified. Lane 7: K4me1. Lane 8: K4me2. Lane 9: K4me3. Lane 10: K4ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

Immunofluorescence using anti-H3T3pK4ac antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4ac is nuclear and chromosomal. Staining: H3T3pK4ac is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight® 550 (red).

Western Blot using anti-phospho-acetyl-Histone H3 antibodies (H3T3pK4ac). Lane 1: HeLa histone extracts. Lane 2. NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 00 for 45 min at RT.
Additional information
Additional information
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
Background
Background
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Phosphorylation of threonine 3 (H3T3p) is a known mitotic marker and modified by the Haspin/Thr3 enzyme, while acetylation of lysine 4 (H3K4ac) on histone 3 is associated with transcriptional activation by Esa1.
Alternative names: H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3pT3/K4ac .
Alternative names: H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3pT3/K4ac .
Product citations
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