H3K4me3T6p | H3 (trimethylated Lys4, p Thr6)

Name: H3K4me3T6p | H3 (trimethylated Lys4, p Thr6)
Brand: IKP
SKU: AS16 3172
Price: 520 EUR
Availability: InStock

AS16 3172 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

H3K4me3T6p | H3 (trimethylated Lys4, p Thr6) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Plant Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3172)

H3K4me3T6p | H3 (trimethylated Lys4, p Thr6)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Caenorhabditis elegans, Human

Predicted reactivity Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Chromatin Immunoprecipitation using anti-H3K4me3T6p antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K4me3T6p antibody alongside a no antibody (No Ab) control, DNA was measured by qPCR and normalized to total input.

Dot Blot using anti-H3K4me3T6p antibodies. Lane 1: Unmodified. Lane 2: T6p. Lane 3: K4Me1T6p. Lane 4: K4Me2T6p. Lane 5: K4Me3T6p. Load: 3, 1, and 0.3 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

IF using anti-H3K4me3T6p antibodies

Immunofluorescence using anti-H3K4me3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me3T6p is nuclear and chromosomal. Staining: H3K4me3T6p is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).

western blot using anti-H3K4me3T6p antibnodies

Western Blot using anti-H3K4me3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Background

Background Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).

Product citations

immunogen:	KLH-conjugated synthetic peptide
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum.
Format:	Liquid
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
calculated \| apparent molecular mass [kDa]:	15 kDa
Confirmed reactivity:	Caenorhabditis elegans, Human
predicted reactivity:	Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Chromatin Immunoprecipitation using anti-H3K4me3T6p antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K4me3T6p antibody alongside a no antibody (No Ab) control, DNA was measured by qPCR and normalized to total input. Dot Blot using anti-H3K4me3T6p antibodies. Lane 1: Unmodified. Lane 2: T6p. Lane 3: K4Me1T6p. Lane 4: K4Me2T6p. Lane 5: K4Me3T6p. Load: 3, 1, and 0.3 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT. Immunofluorescence using anti-H3K4me3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K4me3T6p is nuclear and chromosomal. Staining: H3K4me3T6p is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red). Western Blot using anti-H3K4me3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
additional information:	This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
background:	Methylation of lysine 4 on H3 (H3K4Me) and phosphorylation of threonine 3 (H3T3p) are known marks of transcriptional activation and mitosis. H3K4 has many known modifying enzymes (Set1, Set7/9, MLL, ASH1).