H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8)

Name: H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8)
Brand: IKP
SKU: AS16 3178
Price: 520 EUR
Availability: InStock

AS16 3178 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, Plant, Rat, Xenopus sp.

H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Plant Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3178)

H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8)

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Western blot (WB)

Recommended dilution 2-5 µg/million cells (ChIP), 2 µg/ml (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Caenorhabditis elegans, Human

Predicted reactivity Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

ChIP using anti-H3R8me2(sym) | H3 (sym-dimethyl Arg8) polyclonal antibodies

Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.

western blot using anti-H3R8me2(sym) | H3 (sym-dimethyl Arg8) polyclonal antibodies

Western Blot using anti-H3R8me2(sym) antibodies. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Western Blot using anti-H3R8me2(sym) antibodies. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol

Background

Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, methylation of arginine 8 on histone H3 (H3 R8me2s) is associated with transcriptional repression.

Product citations

immunogen:	KLH-conjugated synthetic peptide
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum.
Format:	Liquid
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Western blot (WB)
recommended dilution:	2-5 µg/million cells (ChIP), 2 µg/ml (WB)
calculated \| apparent molecular mass [kDa]:	15 kDa
Confirmed reactivity:	Caenorhabditis elegans, Human
predicted reactivity:	Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input. Western Blot using anti-H3R8me2(sym) antibodies. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. Western Blot using anti-H3R8me2(sym) antibodies. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
additional information:	This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
background:	Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, methylation of arginine 8 on histone H3 (H3 R8me2s) is associated with transcriptional repression.

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