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H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8)

AS16 3178 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, Plant, Rat, Xenopus sp.
H3R8me2(sym) | H3 Histone 3 (sym-dimethylated Arg8) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3178)

DATA SHEET IN PDF

Qty: 
492
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution 2-5 µg/million cells (ChIP), 2 µg/ml (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Caenorhabditis elegans, Human
Predicted reactivity Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

ChIP using anti-H3R8me2(sym) | H3 (sym-dimethyl Arg8) polyclonal antibodies
Chromatin Immunoprecipitation using the H3R8me2(sym) antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(sym) to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by PCR and normalized to total input.

western blot using anti-H3R8me2(sym) | H3 (sym-dimethyl Arg8)  polyclonal antibodies
Western Blot using anti-H3R8me2(sym) antibodies. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-H3R8me2(sym) | H3 (sym-dimethyl Arg8)  polyclonal antibodies
Western Blot using anti-H3R8me2(sym) antibodies. Lane 1: R8Me2a. Lane 2: R8Me2s. Lane 3: R8Me1. Lane 4: Histone H3 R8. Load: 30 μg per lane. Primary antibody incubated at 2 μg/mL overnight at 4°C. Secondary antibody IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

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collection of antibodies to epigenetics

Background

Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, methylation of arginine 8 on histone H3 (H3 R8me2s) is associated with transcriptional repression.

Product citations

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