IKP

H3T6pK9me3 | Histone H3 (trimethylated Lys9, p Thr6)

Product no: AS16 3176
AS16 3176 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Dot blot (Dot), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 200 (IHC), 1 : 500 (WB)
    Expected | apparent MW:

    15 kDa

  • Reactivity
  • Confirmed reactivity: Caenorhabditis elegans, Human
    Predicted reactivity: Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    dot blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies


    Dot Blot using anti-H3T6p antibodies. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.
    immunofluorescence using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) antibodies
    Immunofluorescence using anti-H3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

    western blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies
    Western Blot using anti-H3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

    western blot using anti-H3T6pK9me3 | Histone H3 (trimethyl Lys9, p Thr6) polyclonal antibodies


    Western Blot of H3T6p antibody on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
  • Additional Information
  • Additional information: This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
  • Background
  • Background: H3K9me3 is a histone post-translational modification that is enriched in transcriptionally inactive regions. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
AS16 3174 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549 €
Add to cart
Info
AS16 3175 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549 €
Add to cart
Info
Close