H3T6pK9me2 | Histone H3 (dimethylated Lys9, p Thr6)

AS16 3175 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 2-5 µg/million cells (ChIP), 1 : 100 (IF), 1 : 200 (IHC), 1 : 500 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Caenorhabditis elegans, Chicken, Drosophila melanogaster, Human, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

ChIP using anti-H3T6pK9me2 | Histone H3 (dimethyl Lys9, p Thr6) polyclonal antibodies

Chromatin Immunoprecipitation using anti-H3T6pK9me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me2 antibody to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

Related products

Background

Background H3K9me2 is a histone post-translational modification that is enriched in transcriptionally inactive regions. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes.