H3T6p | Histone H3 (p Thr6)
AS16 3173 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

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Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Quantity
50 ĩg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 100 (IF), 1 : 200 (IHC), 1 : 500 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Caenorhabditis elegans, Human
Predicted reactivity
Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example
Dot blot using anti-H3T6p antibodies. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.
Immunofluorescence using anti-H3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

Western Blot using anti-H3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Western Blot using anti-H3T6p antibodies on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Dot blot using anti-H3T6p antibodies. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

Immunofluorescence using anti-H3T6p antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

Western Blot using anti-H3T6p antibodies. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Western Blot using anti-H3T6p antibodies on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Additional information
Additional information
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
Background
Background
Phosphorylation as a post-translational modification on histone H3 modulates the ability of proteins to recognize and bind to the H3 tail. When additional modifications to H3 are present, in addition to the native T6 phosphorylation, there is an amplified effect.
Product citations
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