H3R8me2(asym) | Histone H3 (asym-dimethylated Arg8)

AS16 3177 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 2 ug (ChIP), 1: 100 (IF), 1: 100 (IHC), 1:500 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Caenorhabditis elegans, Human

Predicted reactivity Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

ChiP using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies

Chromatin Immunoprecipitation using anti-H3R8me2(asym) antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(asym) and 20ul of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR, and normalized to the input chromatin.

immunofluorescence using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies

Immunofluorescence using anti-H3R8me2(asym) antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: H3R8me2(asym) is nuclear and chromosomal. Staining: H3R8me2(asym) is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).

western blot using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies

western blot using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies

Western Blot using antiH3R8me2(asym) antibodies. Lane 1: HeLa Histone extracts. 2. NIH-3T3 extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

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Background

Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression.