HA, FITC-conjugated (chicken antibody)
AS20 4464 | Clonality: Polyclonal | Host: Chicken | Reactivity: Human influenza hemagglutinin (HA) tagged proteins
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Immunogen KLH-conjugated synthetic petide YPYDVPDYA of influenza virus hemagglutinin.
Purity Affinity purified on antigen
Format Liquid in PBS pH 7.2, containing 0.1% sodium azide.
Quantity 0.1 mg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Flow cytometry (Flowcyt)
Recommended dilution Assay dependent
Confirmed reactivity HA-tagged proteins
Molar F/P ratio is 3.6 and indicates an average number of FITC molecules/IgY molecule as determined by the extinction values at 495 nm and 280 nm (OD495 and OD280), respectively.
Background HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
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