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HDT1 | Histone deacetylase

AS11 1792 | Clonality: Polyclonal | Host: Rabbit | predicted Reactivity: Arabidopsis thaliana

HDT1 | Histone deacetylase  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS11 1792)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

Recombinant part of Arabidopsis thaliana HDT1 Q9FVE6, At3g44750

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid
Quantity 100 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

26.4 kDa

Reactivity

Predicted reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot detection of HDT1 (HD2A)

50 µg of Arabidopsis T87 nuclear proteins, extracted with Nuclei Lysis Buffer (1% SDS, 50mM Tris-HCl, 10mM EDTA, Complete EDTA-free) or 50 µg of total Arabidopsis T87 proteins, extracted with Laemmli Buffer from grinded in liquid nitrogen material, were separated on 12 % SDS-PAGE and blotted 1,5h to PVDF membrane (WestranS 0,20 µm, Whatman). Blots were blocked with 5% fat free milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 2,5% fat-free milk in TBST overnight at 40°C with agitation. The antibody solution was decanted and the blot was washed with TBST three times for 10 min. and blocked in 10% fat-free milk in TBST for 10 min. Next, blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5 000 in 5% fat-free milk in TBST for 2h at RT with agitation. The blot was washed six times for 10 min. with TBST and developed for 5 min with ECL+ (Amersham) according to the manufacturers instructions. Exposure time was 1 min.

Courtesy of Msc. Daniel Buszewicz, PAN, Poland

Additional information

Additional information Antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth

Related products

Background

Background

HDT1 (HD2A) is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in cell cycle progression, transcriptional regulation and developmental events. This protein is also involved in rRNA gene silencing in nucleolar dominance. Synonymes:HD-tuins protein 1, Histone deacetylase 2a.

Product citations

Selected references Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.

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