HDT1 | Histone deacetylase

AS11 1792 | Clonality: Polyclonal | Host: Rabbit | predicted Reactivity: Arabidopsis thaliana

HDT1 | Histone deacetylase  in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS11 1792)
HDT1 | Histone deacetylase


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


Recombinant part of Arabidopsis thaliana HDT1 Q9FVE6, At3g44750

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 26,4 kDa


Predicted reactivity Arabidopsis thaliana
Not reactive in Gossipium sp.

Application examples

Application examples

application example

western blot detection of HDT1 (HD2A)

50 µg of Arabidopsis T87 nuclear proteins, extracted with Nuclei Lysis Buffer (1% SDS, 50mM Tris-HCl, 10mM EDTA, Complete EDTA-free) or 50 µg of total Arabidopsis T87 proteins, extracted with Laemmli Buffer from grinded in liquid nitrogen material, were separated on 12 % SDS-PAGE and blotted 1,5h to PVDF membrane (WestranS 0,20 µm, Whatman). Blots were blocked with 5% fat free milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 2,5% fat-free milk in TBST overnight at 40°C with agitation. The antibody solution was decanted and the blot was washed with TBST three times for 10 min. and blocked in 10% fat-free milk in TBST for 10 min. Next, blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5 000 in 5% fat-free milk in TBST for 2h at RT with agitation. The blot was washed six times for 10 min. with TBST and developed for 5 min with ECL+ (Amersham) according to the manufacturers instructions. Exposure time was 1 min.

Courtesy of Msc. Daniel Buszewicz, PAN, Poland

Additional information

Additional information Antibody is present in PBS + 50 % glycerol and 0,01 % of sodium azide as preservative of bacterial growth

Related products



HDT1 (HD2A) is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in cell cycle progression, transcriptional regulation and developmental events. This protein is also involved in rRNA gene silencing in nucleolar dominance. Synonymes:HD-tuins protein 1, Histone deacetylase 2a.

Product citations

Selected references Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.

Related products: HDT1 | Histone deacetylase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, H. vulg...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS16 3181 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster...
502 €