Horse normal serum (10 ml)

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Product Information
Host
Horse
Purity
Serum
Format
Lyophilized
Quantity
10 ml
Reconstitution
For reconstitution add 11,0 ml of sterile water
Storage
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 šC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Reactivity
Application examples
Additional information
Additional information
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Usually 5 % (v/v) serum blocking buffer it is recommended and for that you need to mix 2 ml of blocking serum with 38 ml of diluent buffer (PBS with TweenŪ 20 detergent) to obtain a total volume of 40 ml, Use immediately or store at 2-8°C or colder,
Background
Background
Normal horse serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. Blocking is a critical step in most immunoassays and "fills-in" the unoccupied spaces of the solid phase that are not occupied by immobilized proteins. Without blocking antibodies would bind non-specifically wich in turn could lead to false signaling and /or background issues.
How to choose blocking serum?
If you use a secondary from donkey e.g. donkey anti-chicken HRP (or other lable) - your blocking serum should be also coming from donkey. Serum from a species primary antibody is coming from should not be used as this will compete for binding sites with secondary antibodies. In this example serum from chicken should not be used as blocking reagent.
How to choose blocking serum?
If you use a secondary from donkey e.g. donkey anti-chicken HRP (or other lable) - your blocking serum should be also coming from donkey. Serum from a species primary antibody is coming from should not be used as this will compete for binding sites with secondary antibodies. In this example serum from chicken should not be used as blocking reagent.