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Horse normal serum (10 ml)

AS10 1562 | Host: horse

For larger volumes, please inquire
Horse normal serum (10 ml) in the group Secondary Antibodies / Blocking/Control / Horse Serum/IgG at Agrisera AB (Antibodies for research) (AS10 1562)

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Product Information

Host Horse
Purity Serum
Format Lyophilized
Quantity 10 ml
Reconstitution For reconstitution add 11.0 ml of sterile water.
Storage Store lyophilized material at 2-8 ºC. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC . For storage at 2-8 ºC, add a preservative to prevent growth of bacteria. Rehydrate with 10 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.

Reactivity

Application examples

Additional information

Additional information Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
Usually 5 % (v/v) serum blocking buffer it is recommended and for that you need to mix 2 ml of blocking serum with 38 ml of diluent buffer (PBS with Tween® 20 detergent) to obtain a total volume of 40 ml. Use immediately or store at 2-8°C or colder. 

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Background

Background Normal horse serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. Blocking is a critical step in most immunoassays and "fills-in" the unoccupied spaces of the solid phase that are not occupied by immobilized proteins. Without blocking antibodies would bind non-specifically wich in turn could lead to false signaling and /or background issues.

How to choose blocking serum?
If you use a secondary from donkey e.g. donkey anti-chicken HRP (or other lable)  - your blocking serum should be also coming from donkey.  Serum from a species primary antibody is coming from should not be used as this will compete for binding sites with secondary antibodies. In this example serum from chicken should not be used as blocking reagent.

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