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Donkey normal serum (2 ml)

AS10 1563 | Host: Donkey

For larger volumes, please inquire
Donkey normal serum (2 ml) in the group Secondary Antibodies / Blocking/Control / Donkey Serum/IgG at Agrisera AB (Antibodies for research) (AS10 1563)

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Product Information

Host Donkey
Purity Serum
Format Lyophilized
Quantity 2 ml
Reconstitution For reconstitution add 2.2 ml of sterile water.
Storage Store lyophilized material at 2-8 ºC. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC . For storage at 2-8 ºC, add a preservative to prevent growth of bacteria. Rehydrate with 2.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.

Reactivity

Application examples

Additional information

Additional information

Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.

Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

Related products

Background

Background Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.

Product citations

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