Donkey normal serum (10 ml)
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Product Information
Host
Donkey
Purity
Serum
Format
Lyophilized
Quantity
10 ml
Reconstitution
For reconstitution add 11,0 ml of sterile water
Storage
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Tested applications
ELISA (ELISA). Immunofluorescence (IF), Western blot (WB)
Reactivity
Application examples
Additional information
Additional information
As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies.
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.
For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies.
Background
Background
Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | 
