Name:
Phone:
E-mail:
Address:






Donkey normal serum (10 ml)

AS10 1564 | Host: Donkey

For larger volumes, please inquire
Donkey normal serum (10 ml) in the group Secondary Antibodies / Blocking/Control / Donkey Serum/IgG at Agrisera AB (Antibodies for research) (AS10 1564)



DATA SHEET IN PDF

Qty: 
193
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Host Donkey
Purity Serum
Format Lyophilized
Quantity 10 ml
Reconstitution For reconstitution add 11,0 ml of sterile water
Storage Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Tested applications ELISA (ELISA). Immunofluorescence (IF), Western blot (WB)

Reactivity

Application examples

Additional information

Additional information

Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.

Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies.

Related products

Background

Background Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.

Product citations

Reconstitution: For reconstitution add 11,0 ml of sterile water
Host: Donkey
Purity: Serum
Format: Lyophilized
Quantity: 10 ml
storage: Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
tested applications: ELISA (ELISA). Immunofluorescence (IF), Western blot (WB)
additional information:

Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.

Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies.
background: Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.

Related products: Donkey normal serum (10 ml)

AS10 1567 | Host: cat

For larger volumes, please inquire
139 €
AS10 1563 | Host: Donkey

For larger volumes, please inquire
139 €
AS10 1547 | Host: Goat

For larger volumes, please inquire
139 €
AS16 3629   | Clonality: Polyclonal |  Host: Pig (swine)

144 €