HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70

AS07 233 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Triticum sp., Pisum sativum

Product Information

Immunogen

HSC70 purified from wheat germ by ATP affinity chromatography

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 50 000 (WB)

Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Cucurbita pepo, Pisum sativum, Triticum sp.

Predicted reactivity Dicots, Monocots

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

This antibody will not react with bip or CSS1. Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70.

This antibody can inhibit protein import into organelles in vitro.

Related products

Collection of antibodies to heat shock proteins

Background

The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

Product citations

Selected references Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242