HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70
AS07 233 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Triticum sp., Pisum sativum
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Product Information
Immunogen
HSC70 purified from wheat germ by ATP affinity chromatography
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 50 000 (WB)
Expected | apparent MW
70 kDa
Reactivity
Confirmed reactivity
Cucurbita pepo, Pisum sativum, Triticum sp.
Predicted reactivity
Dicots, Monocots
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
This antibody will not react with bip or CSS1. Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70.
This antibody can inhibit protein import into organelles in vitro.
Background
Background
The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.
Product citations
Selected references
Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242
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