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HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70

AS07 233  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Triticum sp., Pisum sativum 

HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70 in the group Antibodies Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS07 233)
HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

HSC70 purified from wheat germ by ATP affinity chromatography 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 50 000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Cucurbita pepo, Pisum sativum, Triticum sp.
Predicted reactivity Dicots, Monocots
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information This antibody will not react with bip or CSS1, Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70

This antibody can inhibit protein import into organelles in vitro.

Related products

Background

Background

The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

Product citations

Selected references Koziel et al (2021) Modulation of Expression of PVYNTN RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars. Vaccines. 2021; 9(11):1254. https://doi.org/10.3390/vaccines9111254
Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242
immunogen:

HSC70 purified from wheat germ by ATP affinity chromatography 

Reconstitution: For reconstitution add 100 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 100 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
recommended dilution: 1 : 50 000 (WB)
calculated | apparent molecular mass [kDa]:

70 kDa

Confirmed reactivity: Cucurbita pepo, Pisum sativum, Triticum sp.
predicted reactivity: Dicots, Monocots
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: This antibody will not react with bip or CSS1, Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70
additional information (application):

This antibody can inhibit protein import into organelles in vitro.

background:

The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

All references: Koziel et al (2021) Modulation of Expression of PVYNTN RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars. Vaccines. 2021; 9(11):1254. https://doi.org/10.3390/vaccines9111254
Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242

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