Anti-HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70

Product no: AS07 233

AS07 233  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Triticum sp., Pisum sativum 

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  • Product Info
  • Immunogen:

    HSC70 purified from wheat germ by ATP affinity chromatography 

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 µl
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1 : 50 000 (WB)
    Expected | apparent MW:

    70 kDa

  • Reactivity
  • Confirmed reactivity: Cucurbita pepo, Pisum sativum, Triticum sp.
    Predicted reactivity: Dicots, Monocots
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: This antibody will not react with bip or CSS1, Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70
    Additional information (application):

    This antibody can inhibit protein import into organelles in vitro.

  • Background
  • Background:

    The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

  • Product Citations
  • Selected references: Koziel et al (2021) Modulation of Expression of PVYNTN RNA-Dependent RNA Polymerase (NIb) and Heat Shock Cognate Host Protein HSC70 in Susceptible and Hypersensitive Potato Cultivars. Vaccines. 2021; 9(11):1254. https://doi.org/10.3390/vaccines9111254
    Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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