Name:
Phone:
E-mail:
Message

HSP101 | ClpB heat shock protein, N-terminal (chicken antibody)

AS08 283 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana

HSP101 | ClpB heat shock protein, N-terminal (chicken antibody) in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 283)

DATA SHEET IN PDF

Qty: 
363
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

recombinant Arabidopsis thaliana Hsp101/ClpB, N-terminal 145 amino acids UniProt: P42730,TAIR: At1g74310

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 200 µl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

101 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Nicotiana tabacum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-Hsp101/ClpB antibodies

2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 22ºC, (2) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour followed by a transfer to 22° for 2 hours, (3) Arabidopsis thaliana Hsp101 null mutnat grown at 22°C, (4) Arabidopsis thaliana Hsp101 null mutant stressed at 38ºC for 1.5 hour followed by a transfer to 22ºC for 2 hours were separated on 8 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).

Additional information

Related products

Related products

AS07 253 | Anti-HSP101 | ClpB heat shock protein, N-terminal, rabbit antibodies

AS08 290 | Anti-HSP101 | nuclear/cytoplasmatic heat shock protein, rabbit antibody with reactivity to Zea mays, rabbit antibodies

collection of antibodies to plant heat shock proteins

Background

Background

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

Product citations

Related products: HSP101 | ClpB heat shock protein, N-terminal (chicken antibody)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
185 €
AS07 253 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A. tequilana var. Weber,...
278 €
AS08 290 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stolonifera cv. ‘...
363 €