HSP101 | ClpB heat shock protein, N-terminal (rabbit antibody)
AS07 253 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A. tequilana var. Weber,B. oleracea, N. tabacum, O. sativa, S. lycopersicum, T. aestivum, T. salsuginea, V.faba
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dicots including: Glycine max, Vitis vinifera, monocots
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2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour, (2) Arabidopsis thaliana HSP101 null mutant (hot 1-3) were separated on 7.5% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).
Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 32210987
Journal: Front Plant Sci
Figure Number: 5A
Published Date: 2020-03-27
First Author: Fu, C., Liu, X., et al.
Impact Factor: 5.435Open Publication
The expression of HSPs over the period from heat acclimation to the stage of recovery. Ten-day-old seedlings were first acclimated for 2 h at 38 °C, and then exposed to 44 °C for 2 h, and lastly returned to room temperature (RT) for recovery. At the indicated times (shown at the bottom of the picture), proteins were extracted for western blots. ACTIN was used as a loading control.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.
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