HSP101 | nuclear/cytoplasmatic heat shock protein

AS08 290 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stolonifera cv. ‘Penncross’, Zea mays

Product Information

Immunogen

15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water.

Storage Store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

101 kDa

Reactivity

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Zea mays

Not reactive in Arabidopsis thaliana

Application examples

Additional information

This antibody will not recognize any other cereal hsp101.

Related products

AS07 287 | Anti-HSP101 | ClpB heat shock protein, C-terminal rabbit antibodies

AS08 283 | Anti-HSP101 | ClpB heat shock protein, N-terminal hen antibodies

AS08 290 | Anti-HSP101 | nuclear/cytoplasmatic heat shock protein (Zea mays), rabbit antibodies

collection of antibodies to plant heat shock proteins

Background

Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).

Product citations

Selected references Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).
Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.
Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.