HSP101 | nuclear/cytoplasmatic heat shock protein

Product no: AS08 290

AS08 290 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stolonifera cv. ‘Penncross’Zea mays

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  • Product Info
  • Immunogen:

    15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized at -20C; once reconstituted to a final volume this antibody can be kept in 4C for up to one year, in smaller portions to avoid contamination. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW:

    101 kDa

  • Reactivity
  • Confirmed reactivity: Agostis stolonifera cv. ‘Penncross’, Zea mays
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: Arabidopsis thaliana
  • Additional Information
  • Additional information (application): This antibody will not recognize any other cereal hsp101
  • Background
  • Background:

    Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).

  • Product Citations
  • Selected references: Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
    Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).
    Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.
    Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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