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HSP101 | nuclear/cytoplasmatic heat shock protein

AS08 290 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stolonifera cv. ‘Penncross’, Zea mays

HSP101 | nuclear/cytoplasmatic heat shock protein in the group Antibodies Plant/Algal / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 290)
HSP101 | nuclear/cytoplasmatic heat shock protein



DATA SHEET IN PDF

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302 €
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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

101 kDa

Reactivity

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Zea mays
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in Arabidopsis thaliana

Application examples

Additional information

This antibody will not recognize any other cereal hsp101

Related products

Background

Background

Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).

Product citations

Selected references Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).
Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.
Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
All references: Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).
Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.
Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
calculated | apparent molecular mass [kDa]:

101 kDa
Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822
Purity: Serum
Quantity: 200 ĩl
recommended dilution: 1 : 2000 (WB)
Reconstitution: For reconstitution add 200 ĩl of sterile water
storage: Store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
Confirmed reactivity: Agostis stolonifera cv. ‘Penncross’, Zea mays
predicted reactivity: Species of your interest not listed? Contact us
not reactive in: Arabidopsis thaliana
additional information (application): This antibody will not recognize any other cereal hsp101
background:

Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).

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