ICS1 | Isochorismate synthase 1, chloroplastic

AS16 4107 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICS1 protein sequence, UniProt: Q9S7H8, TAIR: At1g74710.

The peptide is perfectly conserved in ICS1-1 and ICS1-2 isoforms of Arabidopsis thaliana.

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7,4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 62 | 55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana (recombinant ICS1)

Predicted reactivity please inquire

Application examples

Application examples Western blot using anti-ICS1 antibodies

Asterisks indicate ICS1-CFP protein extracted from frozen and ground leaf material of Arabidopsis thaliana adult plants, detected by anti ICS1 antibody (A) and anti-GFP antibody (B). Extraction protocol: total protein was extracted with buffer containing 25mM Tris-HCl pH 8.0, 10mM MgCl2, 1mM DTT, 75 mM NaCl, 0.1% Tween 20, and 20 mM PMSF. 100 µg of protein extract have been loaded in each well of a 10 % SDS-PAGE and blotted for 2h onto PVDF membrane using the wet-blotting technique. Blocking: 2% non-fat milk Primary antibodies: overnight incubation at 4°C with A) anti-ICS1 rabbit polyclonal (Agrisera); dilution 1:1000 and B) anti-GFP mouse monoclonal (Thermo Fisher); dilution 1:5000 Secondary antibodies: 1h incubation with A) goat anti rabbit HRP-conjugated (AS09 602, Agrisera); dilution 1:25 000 at RT, and B) anti-mouse 800CW (Odyssey); dilution 1:20 000 at RT.

Courtesy of Mr. Saad Bin Eusuf and Dr. Julia Krasensky-Wrzaczek; University of Helsinki, Finland

Additional information

ICS1 is present in too low levels to allow detection in control conditions.
Antibody is recognzing ICS1 in transgenic lines which have ICS1 under 35S promotor and is recognizing well YFP-tagged ICS1.

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Collection of antibodies to proteins involved in stress response

Background

Background ICS1 | Isochorismate synthase 1 (chloroplastic) is involved in stress response and defence response to bacterium, fungus, leaf abscission, cold.
Alternative names: AtICS1, EDS16, Enhanced disease susceptibility to erysiphe orontii 16, Salicyic acid induction deficient 2, SID2.