KLH | Keyhole limpet hemocyanin, DyLight® 650 conjugated (40 µg)

Product no: AS99 001-DL650
AS99 001-DL650  |  Clonality: Polyclonal  |  Host: Rabbit | Reactivity: keyhole limpet hemocyanin (KLH), whole molecule
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  • Product Info
  • Immunogen: Purified keyhole limpet hemocyanin (KLH), whole molecule.
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.

    Format: Liquid in PBS pH 7,4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: ca. 400 kDa/subunit
  • Reactivity
  • Confirmed reactivity: KLH protein from Megathura crenulata
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
  • Additional Information
  • Additional information: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al, 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

    DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
  • Background
  • Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa, It is found in the hemolymph of the sea mollusk Megathura crenulata, This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes, Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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