L7/L12 | Ribosomal protein

Name: L7/L12 | Ribosomal protein
SKU: AS08 331
Price: 394 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS08 331 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

L7/L12 | Ribosomal protein in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS08 331)

Product Information

Immunogen

Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1: 10 (IF), 1 : 1000 (WB)

Expected | apparent MW

11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-L7/L12 antibodies

Total protein extracted freshly from Chlamydomonas reinhardtii denatured ay 90°C 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with % milk or % BSA for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed chemiluminescent detection reagent, according to manufacture's recommendations.

Additional information

Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.

Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.

Background

Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463

Product citations

immunogen:	Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	200 µl
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunofluorescence (IF), Western blot (WB)
recommended dilution:	1: 10 (IF), 1 : 1000 (WB)
Expected \| apparent MW:	11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
Confirmed reactivity:	Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Total protein extracted freshly from Chlamydomonas reinhardtii denatured ay 90°C 5 min. were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with % milk or % BSA for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed chemiluminescent detection reagent, according to manufacture's recommendations.
additional information:	Name of this antibody has been changed from L-30 \| 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
additional information (application):	Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
background:	Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463

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