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Anti-LEA4-5 | Late embryogenesis abundant protein 4-5 (serum)
- Product Info
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Immunogen: recombinant LEA4-5 from Arabidopsis thaliana: UniProt: Q9FG31, TAIR: AT5G06760, a group 4 LEA protein (Battaglia et al., 2008)
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 16 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Arachis hypogaea, Brassica sp., Glycine max, Medicago truncatula, Phaseolus vulgaris, Thellungiella halophila
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application information
Arabidopsis thaliana seed protein extracts were obtained as described by Olvera-Carrillo et al. (Plant Physiol 154:373-390, 2010). Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.
Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico - Background
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Background: LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS13 2756 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Phaseolus vulgaris recombiant LEA6-3
AS13 2758A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana