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LEA4-5 | Late embryogenesis abundant protein 4-5 (serum)

AS13 2758 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

LEA4-5 | Late embryogenesis abundant protein 4-5 (serum) in the group Antibodies for Plant/Algal  / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS13 2758)

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Product Information

Immunogen

recombinant LEA4-5 from Arabidopsis thaliana: UniProt: Q9FG31, TAIR: AT5G06760, a group 4 LEA protein (Battaglia et al., 2008)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arachis hypogaea, Brassica sp., Glycine max, Medicago truncatula, Phaseolus vulgaris, Thellungiella halophila
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application information

western blot using anti-AtLEA4-5 antibodies

Arabidopsis thaliana seed protein extracts were obtained as described by Olvera-Carrillo et al. (Plant Physiol 154:373-390, 2010). Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.

Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico

Additional information

Related products

Related products

AS13 2756 | Anti-LEA6-3 | late embryogenesis abundant protein 6-3, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.

Product citations

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