LEA6-1 | Late embryogenesis abundant protein LEA6-1

Product no: AS22 4836

AS22 4836 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana (recombinant protein)

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  • Product Info
  • Immunogen: Recombinant Arabidopsis thaliana LEA6-1, UniProt: O64820,TAIR: AT2G23110
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 500 (WB)
    Expected | apparent MW:

    16 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-LEA6-1 antibodies

    Different amounts (25, 50, 100 and 400 ng) of the purified recombinant AtLEA6-1 protein in Laemmli buffer 1X were denatured at 75°C for 5 min, loaded and separated on 15 % SDS-PAGE. Gel was blotted 1h to nitrocellulose membranes (AmershamTM ProtamTM Premium 0.45 μM NC) using a tank transfer system (BIO-RAD). Blots were blocked ON with TBS-T/NF-Milk (5%) at 8 °C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 1h at RT with agitation in TBS-T/NF-Milk (5%). The antibody solution was decanted, and the blot was rinsed briefly twice with TBS-T and then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/NF-Milk (5%) for 1h at RT with agitation. The blot was washed as above and developed for 3 min with a mix 1:1 of Peroxidase and Luminol (Thermo). Finally, membranes were exposed to X-ray films (Radiographic Blue films, Kodak) for 30’’ and developed using Kodak reagents.

    Courtesy of Dr. Alejandra A. Covarrubias,Universidad Nacional Autónoma de México, Mexico

  • Additional Information
  • Additional information (application): This antibody is recognizing 50 ng of purified, recombinant AtLEA6-1.
  • Background
  • Background:

    LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.

  • Protocols

  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

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