Lhc1 | PS 1 light harvesting holo-complex

AS08 282 | Clonality:Polyclonal | Host:Rabbit | Reactivity:Diatoms

Product Information

Immunogen

Purified LHC complex from Porphyridium cruentum

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

20-25 kDa

Reactivity

Confirmed reactivity Aureococcus anophagefferens, Guillardia theta, Heterosigma akashiwo, Thalassiosira pseudonana, Porphyridium cruentum

Predicted reactivity Diatoms

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana, (2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-Lhc1 (AS08 282, 1:6000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in PBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent. Exposure time was 2 min.

Courtesy of Meriem Alami and Beverley Green, Canada

Additional information

Strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum, reactivity to a few Lhc2 polypeptides of spinach is very low.

Related products

AS09 407 | Anti-Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas, rabbit antibodies

AS09 408 | Anti-Lhcbm5 | chlorophyll a-b binding protein of LHCII, rabbit antibodies

AS06 117 | Anti-Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas, rabbit antibodies

Background

The light-harvesting system of higher plant is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

Product citations

Selected references Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1.
Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566