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Lhc1 | from PSI of red alga

AS08 282  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Diatoms

Lhc1 | from PSI of red alga in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS08 282)

DATA SHEET IN PDF

Qty: 
350
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Purified LHC complex from Porphyridium cruentum

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

20-25 kDa

Reactivity

Confirmed reactivity Aureococcus anophagefferens, Guillardia theta, Heterosigma akashiwo, Thalassiosira pseudonana, Porphyridium cruentum
Predicted reactivity Diatoms and other heterokonts, cryptophyte algae, red algae
Not reactive in Higher plants

Application examples

Application examples

Application example

western blot using anti-Lhc1 antibodies

2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana, (2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-Lhc1 (AS08 282, 1:6000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in PBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent. Exposure time was 2 min.


Courtesy of Meriem Alami and Beverley Green, Canada

Additional information

Strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum

Related products

Background

Background

The light-harvesting system is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

Product citations

Selected references Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1.
Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566

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