showForm Anti-Lhcbm5 | chlorophyll a-b binding protein of LHCII antibody

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Lhcbm5 | Chlorophyll a-b binding protein of LHCII

AS09 408 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

Lhcbm5 | Chlorophyll a-b binding protein of LHCII in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS09 408)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000-1 : 10 000 (WB)
Expected | apparent MW

29 | 30 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity

Chlamydomonas reinhardtii

Application examples

Additional information

Additional information

This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000

This product can be sold containing ProClin in requested.

For western blot detection image please refer to the article below.

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AS09 407 | Anti-Lhcb5 CP26, rabbit antibodies

AS01 011CHLAMYDOMONAS | Set of 4 Chlamydomonas anti-Lhc antibodies (rabbit)

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

Lhcbm5 is one of minor LHCII that associates with PSI in state-2

Product citations

Selected references Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.
Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457.
Jeong et al. (2016). Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2016 Oct 17;1858(1):45-55. doi: 10.1016/j.bbabio.2016.10.007.
Grewe et al. (2014). Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii. Plant Cell. 2014 Apr 4;26(4):1598-1611.

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