m6A | N6-methyladenosine (polyclonal)

AS17 4121 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human, Mouse, other species

Product Information

Immunogen LPH (Limulus polyphemus hemocyanin)- conjugated N6-methyladenosine (m6A)

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Dot blot (Dot)

Recommended dilution 1 : 400 (Dot)

Reactivity

Confirmed reactivity Human, Mouse and other species

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Dot blot using anti-m6A polyclonal antibodies

Dot blot: using anti-m6A rabbit polyclonal antibody with synthetic peptides containing different mofified bases in a range from 50 to 150 pmol) which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A polyclonal antibody (dilution 1:400) in TBS-T with a 1 % non-fat-dry milk.

Additional information

Additional information This antibody is purified on Protein A and provided in PBS pH 7,4 with 0,05 % sodium azide and 0,05 % ProClin300

Background

Background m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA?s, rRNA?s, snRNA?s and in long non-coding RNA?s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.

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Picture (footer):	Application example Dot blot: using anti-m6A rabbit polyclonal antibody with synthetic peptides containing different mofified bases in a range from 50 to 150 pmol) which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A polyclonal antibody (dilution 1:400) in TBS-T with a 1 % non-fat-dry milk.
background:	m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA?s, rRNA?s, snRNA?s and in long non-coding RNA?s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.
Confirmed reactivity:	Human, Mouse and other species
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	This antibody is purified on Protein A and provided in PBS pH 7,4 with 0,05 % sodium azide and 0,05 % ProClin300
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	LPH (Limulus polyphemus hemocyanin)- conjugated N6-methyladenosine (m6A)
Purity:	Total IgG. Protein G purified.
Quantity:	50 µg
recommended dilution:	1 : 400 (Dot)
storage:	Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Dot blot (Dot)