IKP

m6A | N6-methyladenosine (monoclonal)

Product no: AS17 4120
AS17 4120 Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human, Mouse, other species
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  • Product Info
  • Sub class: IgG1
    Immunogen: BSA-conjugated N6-methyladenosine (m6A)
    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG. Protein A purified.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; for long term storage -80C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Dot blot (Dot), RNA Immunoprecipitation (RIP) (RIP)
    Recommended dilution: 1 : 500 (Dot), 1-10 µg (RIP)
  • Reactivity
  • Confirmed reactivity: Human, Mouse and other species
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

     
    Dot blot using anti-m6A antibodies



     
    Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.
  • Additional Information
  • Additional information: This antibody is purified on Protein A and provided in PBS pH 7,4 with 0,05 % sodium azide
  • Background
  • Background: m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.
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