m6A | N6-methyladenosine (monoclonal)
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Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.
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