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m6A | N6-methyladenosine (monoclonal)

AS17 4120 Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Human, Mouse, other species
m6A | N6-methyladenosine (monoclonal) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS17 4120)

DATA SHEET IN PDF

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Product Information

Immunogen BSA-conjugated N6-methyladenosine (m6A)
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein A purified
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Dot blot (Dot), RNA Immunoprecipitation (RIP) (RIP)
Recommended dilution 1 : 500 (Dot), 1-10 µg (RIP)

Reactivity

Confirmed reactivity Human, Mouse and other species
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

 
Dot blot using anti-m6A antibodies



 
Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.

Additional information

Additional information This antibody is purified on Protein A and provided in PBS pH 7.4 with 0.05 % sodium azide.

Related products

Related products AS17 4121 | Anti-m6A | N6-methyladenosine (polyclonal) antibodies

collection of antibodies to epigenetics

Background

Background m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.

Product citations

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