m6A | N6-methyladenosine (monoclonal)

Name: m6A | N6-methyladenosine (monoclonal)
Brand: IKP
SKU: AS17 4120
Price: 393 EUR
Availability: InStock

AS17 4120 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Mouse, other species

m6A | N6-methyladenosine (monoclonal) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Plant Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS17 4120)

Product Information

Immunogen BSA-conjugated N6-methyladenosine (m6A)

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Total IgG. Protein A purified.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Dot blot (Dot), RNA Immunoprecipitation (RIP) (RIP)

Recommended dilution 1 : 500 (Dot), 1-10 µg (RIP)

Reactivity

Confirmed reactivity Human, Mouse and other species

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Dot blot using anti-m6A antibodies

Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.

Additional information

Additional information This antibody is purified on Protein A and provided in PBS pH 7,4 with 0,05 % sodium azide

Background

Background m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.

Product citations

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immunogen:	BSA-conjugated N6-methyladenosine (m6A)
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Total IgG. Protein A purified.
Format:	Liquid
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; for long term storage -80°C is recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Dot blot (Dot), RNA Immunoprecipitation (RIP) (RIP)
recommended dilution:	1 : 500 (Dot), 1-10 µg (RIP)
Confirmed reactivity:	Human, Mouse and other species
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Dot blot: using anti-m6A mouse monoclonal antibody with DNA oligonucleotide containing different m6A modified bases (in a range from 4 to 100 pmol) and a negative control which were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the mouse anti-m6A monoclonal antibody (dilution 1:500) in TBS-T with a 10 % non-fat-dry milk.
additional information:	This antibody is purified on Protein A and provided in PBS pH 7,4 with 0,05 % sodium azide
background:	m6A (N6-methyladenosine) is a modified base, abundant in mRNA in most eukaryotes and found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU.