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mCherry (rabbit antibody)

AS18 4179 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mCherry

mCherry (rabbit antibody) in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS18 4179)

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Product Information

Immunogen Recombinant full length mCherry expressed and purified from E. coli.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified in PBS pH 7.4, contains 3% trehalose.
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

Reactivity

Confirmed reactivity mCherry

Application examples

Application examples application example


A: Western blot  of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (AS18 4179). 

Additional information

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.

Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.

Related products

Related products

AS16 3967 | Anti-mCherry, monoclonal antibodies
AS18 4178 | Anti-mCherry, chicken antibodies
AS15 2987
| Anti-GFP (affinity purified), rabbit antibodies
AS15 2999 | Anti-GFP (total IgG), rabbit antibodies
AS15 3000 | Anti-GFP (protein A purified) rabbit antibodies
AS15 2998 | Anti-GFP (total IgY), chicken antibodies

AS15 3002 | GFP (purified recombinant protein)
other tag antibodies

Background

Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).

Product citations

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