1

mCherry (rabbit antibody)
AS18 4179 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mCherry
- Product Info
-
Immunogen: Recombinant full length mCherry expressed and purified from E. coli. Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified in PBS pH 7.4, with 3% trehalose. Format: Lyophilized Quantity: 100 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocytochemistry (ICC), Western blot (WB) Recommended dilution: 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB) - Reactivity
-
Confirmed reactivity: mCherry Predicted reactivity: mScarlett - Application Examples
-
application example
A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (AS18 4179).
- Additional Information
-
Additional information (application): In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot. - Background
-
Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). - Reviews:
-
To leave a review, you should have previously purchased this product and log in to your customer profile.
This product doesn't have any reviews.
Accessories

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right