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Anti-mCherry (rabbit antibody)
Product no: AS18 4179
AS18 4179 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mCherry
361
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: Recombinant full length mCherry expressed and purified from E. coli. Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified in PBS pH 7.4, with 3% trehalose. Format: Lyophilized Quantity: 100 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocytochemistry (ICC), Western blot (WB) Recommended dilution: 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB) - Reactivity
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Confirmed reactivity: mCherry Predicted reactivity: mScarlett - Application Examples
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application example 
A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (AS18 4179).
- Additional Information
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Additional information (application): In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot. - Background
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Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). - Product Citations
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Selected references: Song et al. (2024). Substrate-induced condensation activates plant TIR domain proteins.Nature. 2024 Mar 13.doi: 10.1038/s41586-024-07183-9. - Reviews:
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