mCherry (rabbit antibody)

Name: mCherry (rabbit antibody)
Brand: IKP
SKU: AS18 4179
Price: 331 EUR
Availability: InStock

AS18 4179 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mCherry

mCherry (rabbit antibody) in the group Tag Antibodies / mCherry/mNeonGreen/mStrawberry at Agrisera AB (Antibodies for research) (AS18 4179)

Product Information

Immunogen Recombinant full length mCherry expressed and purified from E. coli.

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

Reactivity

Confirmed reactivity mCherry

Application examples

Application examples application example

A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (AS18 4179).

Additional information

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.

Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.

Background

Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).

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additional information (application):	In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector. Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.
Confirmed reactivity:	mCherry
background:	mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Picture (footer):	application example A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2). B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (AS18 4179).
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant full length mCherry expressed and purified from E. coli.
Purity:	Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.
Quantity:	100 µg
recommended dilution:	1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunocytochemistry (ICC), Western blot (WB)