mCherry (chicken antibody)
AS18 4178 | Clonality: Polyclonal | Host: Chicken | Reactivity: mCherry

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Product Information
Immunogen
Recombinant full length mCherry expressed and purified from E. coli.
Host
Chicken
Clonality
Polyclonal
Purity
Concentrated IgY preparation.
Format
Lyophilized with 3% trehalose.
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
1 : 1000 (ICC), 1 : 2000-1 : 5000 (WB)
Reactivity
Confirmed reactivity
mCherry
Application examples
Application examples
Application example

A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (chicken antibody) AS18 4178.

A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (chicken antibody) AS18 4178.
Additional information
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
Background
Background
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Product citations
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