mCherry (chicken antibody)

AS18 4178 | Clonality: Polyclonal | Host: Chicken | Reactivity: mCherry

Product Information

Immunogen Recombinant full length mCherry expressed and purified from E. coli.

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY (chicken egg yolk immunoglobulin) with 3% trehalose.

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 1000 (ICC), 1: 2000 (IF), 1 : 2000-1 : 5000 (WB)

Reactivity

Confirmed reactivity mCherry

Application examples

Application examples Application example

A: Western blot of HEK293 cells transfected with pFin-EF1-mCherry vector. Transfected cells (1). Non-transfected control cells (2).
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells using anti-mCherry (chicken antibody) AS18 4178.

Additional information

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector

Related products

AS16 3967 | Anti-mCherry, mouse monoclonal antibodies
AS15 2987 | Anti-GFP (affinity purified), rabbit antibody
AS15 2999 | Anti-GFP (total IgG), rabbit antibody
AS15 3000 | Anti-GFP (protein A purified) rabbit antibody
AS15 2998 | Anti-GFP (total IgY), chicken antibody

AS15 3002 | GFP (purified recombinant protein)
other tag antibodies

Background

Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).