mCherry (monoclonal)

AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry


product information
Background		mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Immunogen		Recombinant full length mCherry expressed and purified from E. coli.
Host		Mouse
Clonality		Monoclonal
Clone		IgG2a
Purity		Purified from tissue culture supernatant.
Format		Lyophilized
Quantity		100 µg
Reconstitution		For reconstitution add 100 µl of sterile water
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunocytochemistry (ICC) (IC), Western blot (WB)
Related products		AS15 2987 \| GFP (affinity purified), rabbit antibody AS15 2999 \| GFP (total IgG), rabbit antibody AS15 3000 \| GFP (protein A purified) rabbit antibody AS15 2998 \| GFP (total IgY), chicken antibody AS15 3002 \| GFP (purified recombinant protein) other tag antibodies
Additional information

application information
Recommended dilution		1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Expected \| apparent MW
Confirmed reactivity		mCherry
Predicted reactivity
Not reactive in
Additional information		In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
Selected references		to be added when available, antibody released in August 2016.

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