Anti-mCherry (monoclonal)
Product no: AS16 3967
AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry
361
€
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- Product Info
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Immunogen: Recombinant full length mCherry expressed and purified from E. coli. Sub class: IgG2a Host: Mouse Clonality: Monoclonal Purity: IgG2a purified from tissue culture supernatant. Format: Lyophilized Quantity: 100 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunocytochemistry (ICC) (IC), Western blot (WB) Recommended dilution: 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB) - Reactivity
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Confirmed reactivity: mCherry Predicted reactivity: mScarlett - Additional Information
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Additional information (application): In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector - Background
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Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). - Product Citations
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Selected references: Xie et al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033. - Reviews:
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