mCherry (monoclonal)
AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry

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Product Information
Immunogen
Recombinant full length mCherry expressed and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG2a
Purity
IgG2a purified from tissue culture supernatant.
Format
Lyophilized
Quantity
100 ĩg
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunocytochemistry (ICC) (IC), Western blot (WB)
Recommended dilution
1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Reactivity
Confirmed reactivity
mCherry
Application examples
Additional information
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector
Background
Background
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Product citations
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