mCherry (monoclonal)

AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry

Product Information

Immunogen Recombinant full length mCherry expressed and purified from E. coli.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2a

Purity IgG2a purified from tissue culture supernatant.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunocytochemistry (ICC) (IC), Western blot (WB)

Recommended dilution 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)

Reactivity

Confirmed reactivity mCherry

Application examples

Additional information

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector

Background

Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).