IKP

mCherry (monoclonal)

Product no: AS16 3967

AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry

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  • Product Info
  • Immunogen: Recombinant full length mCherry expressed and purified from E. coli.
    Sub class: IgG2a
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG2a purified from tissue culture supernatant.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocytochemistry (ICC) (IC), Western blot (WB)
    Recommended dilution: 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
  • Reactivity
  • Confirmed reactivity: mCherry
    Predicted reactivity: mScarlett
  • Additional Information
  • Additional information (application): In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector
  • Background
  • Background: mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
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