mCherry (monoclonal)

AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry

mCherry (monoclonal) in the group Human/Animal Antibodies / Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS16 3967)


313 €

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product information
Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Immunogen Recombinant full length mCherry expressed and purified from E. coli.
Host Mouse
Clonality Monoclonal
Clone IgG2a
Purity Purified from tissue culture supernatant.
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunocytochemistry (ICC) (IC), Western blot (WB)
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Additional information
application information
Recommended dilution 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Expected | apparent MW
Confirmed reactivity mCherry
Predicted reactivity
Not reactive in
Additional information In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector. 
Selected references

to be added when available, antibody released in August 2016. 

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