AS16 3967 | Clonality: Monoclonal | Host: Mouse | Reactivity: mCherry
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Immunogen Recombinant full length mCherry expressed and purified from E. coli.
Purity Purified from tissue culture supernatant.
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunocytochemistry (ICC) (IC), Western blot (WB)
Recommended dilution 1 : 250-1 : 500 (IC), 1 : 1000-1 : 2000 (WB)
Confirmed reactivity mCherry
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.
Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
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