IKP

MEB2 | Membrane protein of ER body 2

Product no: AS20 4422
AS20 4422 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
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  • Product Info
  • Immunogen: Purified recombinant MEB2 of Arabidopsis thaliana, residues 1-325 with a His tag, UniProt: F4KFS7 , TAIR: At5g24290
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
    Format: Liquid at 2 mg/ml.
    Quantity: 200 g
    Storage: Store at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1:1000 - 1: 2000 (IHC), 1: 10 000 (WB)
    Expected | apparent MW: 61 | 82 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-MEB2 antibodies

    Arabidopsis thaliana 7 day seedling crude extract was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Protein load/well is 10 µg. Sample was separated on 7.5 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.
    Calculated MW of MEB2 is 61 kDa, while apparent MW appears to be 82 kDa (ER membrane protein).
  • Additional Information
  • Additional information (application): Due to N-linked glycosylation at 9 asparagine residues 19-amino acids are cut of as signal peptide from N-terminus of MEB2,
  • Background
  • Background: MEB2 (Membrane protein of ER body 2) may sequester excess cytosolic iron and manganese into endoplasmic reticulum to reduce metal ion toxicity. Not essential for the accumulation of ER body components, including PYK10.
  • Product Citations
  • Selected references: Yamada et al. (2013). Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. Plant Physiol . 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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