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Anti-MKKK18 | Mitogen-activated protein kinase 18
AS13 2673 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Data sheet
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- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana kinase 18 sequence UniProt: Q9ZVP5, TAIR: AT1G05100
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 3 µl (IP), 1 : 1000 (WB) Expected | apparent MW: 37.7 | 38 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
Various samples from Arabidopsis thaliana cells overexpressing MKK18 were separated on 12% SDS-PAGE and blotted 1h to PVDF (semi-dry). Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blot was incubated with the primary antibody diluted to 1: 1000 for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:50 000 in for 30min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 5 min.
Courtesy of Malgorzata Tajdel, Ludwików Lab, Adam Mickiewicz University, Poland - Additional Information
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Additional information (application): MKKK18 is not stable in endogenous extracts and to allow succesfull detection use transgenic plants or transient expression in protoplasts - Background
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Background: Mitogen-activated protein kinase 18 has ATP binding and serine/threonine kinase activity. Alternative names: T7A14.2 protein, Putative NPK1-related MAP kinase.
- Product Citations
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Selected references: Tajdel-Zielińska et al. (2023). Arabidopsis HECT and RING-type E3 Ligase Promote MAPKKK18 Degradation To Regulate Abscisic Acid Signalling. Plant Cell Physiol. 2023 Dec 28:pcad165.doi: 10.1093/pcp/pcad165.
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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