MKKK18 | Mitogen-activated protein kinase 18

KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana kinase 18 sequence UniProt: Q9ZVP5, TAIR: AT1G05100

Various samples from Arabidopsis thaliana cells overexpressing MKK18 were separated on 12% SDS-PAGE and blotted 1h to PVDF (semi-dry). Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blot was incubated with the primary antibody diluted to 1: 1000 for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:50 000 in for 30min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 5 min.

Courtesy of Malgorzata Tajdel, Ludwików Lab, Adam Mickiewicz University, Poland

Mitogen-activated protein kinase 18 has ATP binding and serine/threonine kinase activity. Alternative names: T7A14.2 protein, Putative NPK1-related MAP kinase.