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MKKK18 | Mitogen-activated protein kinase 18

AS13 2673   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

MKKK18 | Mitogen-activated protein kinase 18 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS13 2673)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana kinase 18 sequence UniProt: Q9ZVP5, TAIR: AT1G05100

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 3 µl (IP), 1 : 1000 (WB)
Expected | apparent MW

37.7 | 38 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-MKK18 antibodies

Various samples from Arabidopsis thaliana cells overexpressing MKK18 were separated on 12% SDS-PAGE and blotted 1h to PVDF (semi-dry). Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blot was incubated with the primary antibody diluted to 1: 1000 for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:50 000 in for 30min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 5 min.

Courtesy of Malgorzata Tajdel, Ludwików Lab, Adam Mickiewicz University, Poland

Additional information

MKKK18 is not stable in endogenous extracts and to allow succesfull detection use transgenic plants or transient expression in protoplasts.

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Background

Background

Mitogen-activated protein kinase 18 has ATP binding and serine/threonine kinase activity. Alternative names: T7A14.2 protein, Putative NPK1-related MAP kinase.

Product citations

Selected references Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.

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