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MnSOD3 | Superoxide dismutase (Algal)

AS21 4523 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Chlamydomonas reinhardii
MnSOD3 | Superoxide dismutase (Algal) in the group Antibodies for Plant/Algal  / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS21 4523)

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Product Information

Immunogen Recombinant MnSOD3 of Chlamydomonas reinhardtii, product of a MSD3 gene Cre16.g676150; Phytozome
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 32 | 35 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity green algae
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Specific extraction method requires to be applied, check as published in Page et al. (2012).
MnSOD3  can be only detected in Fe-limited cells (0.5 or 0.2 mM added Fe) (i.e., samples exhibiting the novel MnSOD activity) but not in cells grown with higher concentrations of added Fe.

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Background

Background MnSOD3 (Superoxide dismutase) is an chloroplastic enzyme which destroys radicals which are normally produced within the cells and which are toxic to biological systems. It is induced under Fe limitation, Mn Deficiency, and H2O2 stress as shown by Page et al. (2012).

Product citations

Selected references Page et al. (2012) Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii. Plant Cell. 2012 Jun;24(6):2649-65. doi: 10.1105/tpc.112.098962. Epub 2012 Jun 8. PMID: 22685165; PMCID: PMC3406916.

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