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MT2a | Metallothionein 2a

AS13 2685  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare

MT2a | Metallothionein 2a in the group Antibodies for Plant/Algal  / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS13 2685)

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Hordeum vulgare MT2a sequence, UniProt I6NQG8

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 1000 (WB) on recombinant protein, 1 : 400 (IL)
Expected | apparent MW

7.7 kDa

Reactivity

Confirmed reactivity Horderum vulgare
Predicted reactivity Horderum vulgare
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

This antibody has so far worked on recombiant protein in western blot.

Immunolocalization experiment is described in detail in Schiller et al. (2013).

Related products

Related products

AS13 2684 | Anti-MT1a | Metallothioneine type1, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

MT2a (Metallothionein 2a) is a cysteine-rich, low molecular mass protein with a metal ion binding function.  Metallothionenins (MTs) are involved in many physiological processes such as metal ion homeostasis, detoxification of excess metals and protection against oxidative stress.

Product citations

Selected references Schiller et al. (2013). Barley metallothioneins differ in ontogenetic pattern and response to metals. Plan Cell Environ June 28.

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