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Anti-Myc epitope tag
Product no: AS21 4685
AS21 4685 | Clonality: Monoclonal | Host: Mouse | Reactivity: Myc-tagged proteins
169
€
Customer reviews
Delivery:
3-6 business days
- Product Info
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Immunogen: Myc epitope tag, sequence: EQKLISEEDL Sub class: IgG1a Host: Mouse Clonality: Monoclonal Purity: Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol. Format: Liquid Quantity: 50 µg Storage: Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1: 500 - 1: 5000 (WB) Expected | apparent MW: Depends upon protein fusion partner - Reactivity
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Confirmed reactivity: Myc epitope tag - Application Examples
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20 µL of 150 µg/mL protein extract from nuclear fraction of A. thaliana dry seeds using the commercial buffer from BIORAD, laemmli 4x ref 1610747. Extracts were denatured for 5 min at 90°C and subjected to electrophoresis on BIORAD gradient gel (4-20%), mini-PROTEAN TGX gel (ref 4561093) at room temperature. The transfer was done for 7 minutes with the BIORAD semi-dry trans-blot turbo kit on nitrocellulose with the pore size of 0.2 µm. The blot was blocked with 5% of nonfat milk in PBST for 1h/RT. The blot was incubated with primary antibody at the dilution of 1:1000 ON/4°C with agitation, in 3% milk in PBST. The blot was washed 3 times for 5 minutes in PBST, under agitation at RT. The secondary antibody was diluted to 1:5000 dilution, and incubated for 2 h/RT with agitation in 3% nonfat milk in PBST. The blot was developed using chemiluminescent detection reagnet according to manufacture's recommendations.Courtesy of Dr. Lauriane Simon, iGReD, France
- Background
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Background: The anti-Myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc. - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody Protocols - Reviews:
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