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NAI2 TSA1-like protein, C-terminal

AS20 4420 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana
NAI2  TSA1-like protein, C-terminal in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS20 4420)

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Product Information

Immunogen Purified recombinant C-terminal part of NAI2 of Arabidopsis thaliana, residues 636-772 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Quantity 200 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1: 2000 - 1: 4000 (WB)
Expected | apparent MW 85 | 120 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-NAI2 antibodies
Arabidopsis thaliana 7 day seedling extract was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Sample was separated on 12.5 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

The nature of LMW band was not investigated.

Additional information

Additional information Signal peptide of 24 amino acids is removed from the mature protein.

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Background

Background NAI2 TSA1-like protein, C-terminal is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Localised to ER lumen.

Product citations

Selected references Yamada et al. (2008). NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20(9):2529-40. doi: 10.1105/tpc.108.059345.

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