NAI2 TSA1-like protein, C-terminal

Name: NAI2 TSA1-like protein, C-terminal
Brand: IKP
SKU: AS20 4420
Price: 349 EUR
Availability: InStock

AS20 4420 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

NAI2 TSA1-like protein, C-terminal in the group Antibodies Plant/Algal / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS20 4420)

Product Information

Immunogen Purified recombinant C-terminal part of NAI2 of Arabidopsis thaliana, residues 636-772 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 2 mg/ml.

Quantity 200 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 2000 - 1: 4000 (WB)

Expected | apparent MW 85 | 120 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-NAI2 antibodies

Arabidopsis thaliana 7 day seedling extract was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Sample was separated on 12.5 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

The nature of LMW band was not investigated.

Additional information

Additional information Signal peptide of 24 amino acids is removed from the mature protein

Background

Background NAI2 TSA1-like protein, C-terminal is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Localised to ER lumen.

Product citations

Selected references Yamada et al. (2008). NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20(9):2529-40. doi: 10.1105/tpc.108.059345.

calculated \| apparent molecular mass [kDa]:	85 \| 120 kDa
Clonality:	Polyclonal
Format:	Liquid at 2 mg/ml.
Host:	Rabbit
immunogen:	Purified recombinant C-terminal part of NAI2 of Arabidopsis thaliana, residues 636-772 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Quantity:	200 µg
recommended dilution:	1: 2000 - 1: 4000 (WB)
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
background:	NAI2 TSA1-like protein, C-terminal is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Localised to ER lumen.
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Signal peptide of 24 amino acids is removed from the mature protein
All references:	Yamada et al. (2008). NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20(9):2529-40. doi: 10.1105/tpc.108.059345.
Picture (footer):	Arabidopsis thaliana 7 day seedling extract was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Sample was separated on 12.5 % SDS-PAGE and blotted at 15V overnight using wet transfer to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation. The nature of LMW band was not investigated.

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