NdbA | Thylakoid Localized Type 2 NAD(P)H Dehydrogenase

AS19 4297 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 680

NdbA | Thylakoid Localized Type 2 NAD(P)H Dehydrogenase

Product Information

Immunogen KLH-conjugated peptide derived from NdbA protein sequence of Synechocystis sp. PCC 6803, UniProt:P73739
Chosen peptide is not conserved in NdbB and NdbC.

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW 49 | 46 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 68

Predicted reactivity Bacillus subtilis
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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Protein extraction: harvested cells were suspended in a buffer containing 50 mM Hepes-NaOH, pH 7,5, 30 mM CaCl2, 800 mM sorbitol, 1 mM ε-amino-n-caproic acid, and the cells were broken by vortexing 6×1 min at 4°C in the presence of glass beads, Protein samples were solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea

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Background

Background NdbA (Thylakoid Localized Type 2 NAD(P)H Dehydrogenase) is localized to the thylakoid membrane and expressed at a very low level in the widl type under photoautotrophic growth. NdbA is induced substantially under light-activated heterotophic growth conditions.

Product citations

Selected references Huokko et al. (2019). Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol. 2019 Mar 7. pii: pcz044. doi: 10.1093/pcp/pcz044.