FDX1 | Ferredoxin
AS06 121 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, P. strobus, S. oleracea, Ch. reinhardtii, Synechocystis 6803 substrain PCC-M
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Native ferredoxin purified from Spinacia oleracea.
10 (Spinacia oleracea); 16.7 (Arabidopsis thaliana), 13.7 (Chlamydomonas reinhardtii)
Dicots, Nicotiana tabacum, Physcomitrella patens, Zea mays
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20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 % acrylamide gel with 6 M urea. Filters were blotted on PVDF, blocked (1 h) with 5 % milk, incubated with 1: 1000 anti-ferredoxin (over night in 4ºC) in 1 % milk/TBS-T) followed by incubation with 1: 10000 secondary antibody (2 h) coupled to HRP and visualization with standard ECL.
20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 % acrylamide gel with 6 M urea. Filters were blotted on PVDF,5 µg of soluble protein extracts from Chlamydomonas reinhardtii strain CC-4532 were separated on 15 % SDS-PAGE and blotted at 4 W for 30 min to nitrocellulose membrane (0.1 µm pore size) using semi-dry transfer. The blot was blocked with 1 % non-fat dry milk overnight at room temperature (RT) with agitation. The blot was incubated in the primary antibody solution at a dilution of 1: 10 000 in PBS-T+1% milk for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. The blot was incubated in secondary antibody solution (anti-rabbit IgG-AP conjugated) diluted to 1: 300 in PBS-T+1% milk for 1h at RT with agitation. The blot was washed as above and developed with exposure time of 2.5 minutes.
Courtesy of Dr. Marcus Miethke, Department of Chemistry & Biochemistry, UCLA
In Arabidopsis thaliana leaf extracts there is a strong cross-reactivity at 20 kDa.
Load per well: 20-40 µg/lane required for Arabidopsis thaliana, 2-10 µg for other species.
This product can be sold containing proclin if requested.
Ferredoxins are acidic, low molecular weight, soluble iron-sulfur proteins found in various organisms. Iron-sulfur proteins are defined as proteins carrying iron-sulfur cluster(s) in which the iron is at least partially coordinated by sulfur. The protein acts as multifunctional electron carriers in diverse redox systems. The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis and other reductive reactions in the chloroplast.
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