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Non-fucosylated xyloglucan-3 (clone CCRC-M100)

AS16 3234-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Non-fucosylated xyloglucan-3 (clone CCRC-M100) in the group Antibodies Plant/Algal / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3234-1ml)
Non-fucosylated xyloglucan-3 (clone CCRC-M100)



DATA SHEET IN PDF

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Data sheet Product citations Add review

Product Information

Immunogen BSA-conjugated sycamore ((Acer pseudoplatanus)) xyloglucan, covalent binding.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Reactivity

Confirmed reactivity Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus
Predicted reactivity Dicots
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user
CCRC-M100 binds only to the xyloglucan sub-unit, XXXG, and shows no cross-reactivity with other xyloglucan sub-units tested, CCRC-M100 shows some cross-reactivity with sycamore pectic polysaccharides and linseed mucilage

Related products

Background

Background Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody binds to galactosylated side-chains of non-fucosylated xyloglucan-3, and appears to preferentially bind to the galactosylated side-chain closest to the reducing end of xyloglucan oligosaccharide sub-units (XXXG).

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen: BSA-conjugated sycamore ((Acer pseudoplatanus)) xyloglucan, covalent binding.
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgM
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
background: Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody binds to galactosylated side-chains of non-fucosylated xyloglucan-3, and appears to preferentially bind to the galactosylated side-chain closest to the reducing end of xyloglucan oligosaccharide sub-units (XXXG).
additional information: Exact working dilution needs to be determined by end user
additional information (application): CCRC-M100 binds only to the xyloglucan sub-unit, XXXG, and shows no cross-reactivity with other xyloglucan sub-units tested, CCRC-M100 shows some cross-reactivity with sycamore pectic polysaccharides and linseed mucilage
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Confirmed reactivity: Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus
predicted reactivity: Dicots
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known