NtcA | Global nitrogen regulator

AS12 1873 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria

20 % until end of March 2023. Use discount code: Nitro20

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including UniProt: P33779

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

25 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC6803

Predicted reactivity Anabaena sp., Gleobacter sp., marine Synechococcus strains, Trichodesmium sp.
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

western blot detection of NtcA

5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.

Additional information

Background

NtcA acts as a transcriptional activator of genes subjected to nitrogen control.

Product citations

Selected references Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.

Confirmed reactivity:	Synechocystis sp. PCC6803
predicted reactivity:	Anabaena sp., Gleobacter sp., marine Synechococcus strains, Trichodesmium sp. Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
All references:	Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.
background:	NtcA acts as a transcriptional activator of genes subjected to nitrogen control.
calculated \| apparent molecular mass [kDa]:	25 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including UniProt: P33779
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	200 µg
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Picture (footer):	application example 5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.