NtcA | Global nitrogen regulator
AS12 1873 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
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5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.
NtcA acts as a transcriptional activator of genes subjected to nitrogen control.
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