OLE2 | Oleosin 21,2 kDa

Name: OLE2 | Oleosin 21,2 kDa
Brand: IKP
SKU: AS20 4411
Price: 349 EUR
Availability: InStock

AS20 4411 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

OLE2 | Oleosin 21,2 kDa in the group Antibodies Plant/Algal / Plant Developmental Biology / Plant Signal Transduction at Agrisera AB (Antibodies for research) (AS20 4411)

Product Information

Immunogen Conjugated peptide, derived from Arabidopsis thaliana oleosin OLE2, UniProt: Q39165, TAIR: At5g40420

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 2 mg/ml.

Quantity 200 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1: 10 000 - 1: 20 000 (WB)

Expected | apparent MW 21 | 19 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Camelina sativa, Capsella rubella, utrema salsugineum. Raphanus sativus Species of your interest not listed? Contact us

Not reactive in Glycine max

Application examples

Application examples Western blot using anti-oleosin 21.2 kDa antibodies

Homogenates of dry seeds of Arabidopsis thaliana wild-type (left panel), oleosin-deficient mutants: ole4, ole3, ole1 and ole2 (from left to right following wild-type) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15 % SDS-PAGE and blotted to PVDF membrane. Blot was blocked with 5 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

Additional information

Background

Background Oleosins may have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth. Oleosins also increase the viability of over-wintering oilseeds by preventing abnormal fusion of oil bodies during imbibition in the spring. Cellular localisation: surface of oil bodies.

Product citations

Selected references Shimada et al. (2008). A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thaliana. Plant J. 2008 Sep;55(5):798-809. doi: 10.1111/j.1365-313X.2008.03553.x.

immunogen:	Conjugated peptide, derived from Arabidopsis thaliana oleosin OLE2, UniProt: Q39165, TAIR: At5g40420
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format:	Liquid at 2 mg/ml.
Quantity:	200 µg
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1: 10 000 - 1: 20 000 (WB)
calculated \| apparent molecular mass [kDa]:	21 \| 19 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Camelina sativa, Capsella rubella, utrema salsugineum. Raphanus sativus Species of your interest not listed? Contact us
not reactive in:	Glycine max
Picture (footer):	Homogenates of dry seeds of Arabidopsis thaliana wild-type (left panel), oleosin-deficient mutants: ole4, ole3, ole1 and ole2 (from left to right following wild-type) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15 % SDS-PAGE and blotted to PVDF membrane. Blot was blocked with 5 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
background:	Oleosins may have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth. Oleosins also increase the viability of over-wintering oilseeds by preventing abnormal fusion of oil bodies during imbibition in the spring. Cellular localisation: surface of oil bodies.
All references:	Shimada et al. (2008). A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thaliana. Plant J. 2008 Sep;55(5):798-809. doi: 10.1111/j.1365-313X.2008.03553.x.