OLE2 | Oleosin 21,2 kDa
|Data sheet||Product citations||Protocols||Add review|
Homogenates of dry seeds of Arabidopsis thaliana wild-type (left panel), oleosin-deficient mutants: ole4, ole3, ole1 and ole2 (from left to right following wild-type) were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 15 % SDS-PAGE and blotted to PVDF membrane. Blot was blocked with 5 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
Related products: OLE2 | Oleosin 21,2 kDa
This product can b...