PAL 1-4 | Phenylalanine ammonia-lyase 1-4

Name: PAL 1-4 | Phenylalanine ammonia-lyase 1-4
SKU: AS21 4614
Price: 319 EUR
Availability: InStock

Product no: AS21 4614

AS21 4614 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana benthamiana

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DATA SHEET IN PDF

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Product Info

Immunogen:	KLH-conjugated, conserved peptide derived from Arabidopsis thaliana PAL1-4, UniProt: P35510, P45724, P45725, Q9SS45 , TAIR:AT2G37040, AT3G53260, AT5G04230 ,AT3G10340
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Antigen affinity purified serum, in PBS pH 7.4
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl, of sterile or deionized water.
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 2000 (WB)
Expected \| apparent MW:	76.2-78.7 kDa

Reactivity

Confirmed reactivity:	Nicotiana benthamiana
Predicted reactivity:	Arabidopsis thaliana, Hibiscus syriacus, Lotus corniculatus, Solanum dulcamara, Solanum lycopersicum,Vigna unguiculata, Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Western blot using anti phenylalanine ammonia-lyase 1-4

Target MW: ~77 kDa

Target cellular localisation: Cytoplasmic

~25 µg/well of total protein extracted freshly from N. benthamiana leaf. Sample 3 is a control, no overexpression. Exact buffer components were: Laemmli buffer (62.5mM Tris-HCl (pH 6.8), 10% glycerol, 1%SDS, 0.005% Bromophenol Blue): and denatured with exact buffer components at 70°C for 10 min. Samples were separated on 10 % SDS-PAGE and blotted for 0.5 h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:2 000 in TBS-T ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10 AgriseraBright (mid picogram detection). Exposure time was 5 minutes (LI-COR Odyssey FC Imaging System).

Image courtesy of Dr Yasin Tumtas, Bozkurt Lab, Imperial College London

Background

Background:

This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Poster Collection
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