PAL 1-4 | Phenylalanine ammonia-lyase 1-4
AS21 4614 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana benthamiana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated, conserved peptide derived from Arabidopsis thaliana PAL1-4, UniProt: P35510, P45724, P45725, Q9SS45 , TAIR:AT2G37040, AT3G53260, AT5G04230,AT3G10340
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
76.2-78.7 kDa
Reactivity
Confirmed reactivity
Nicotiana benthamiana
Predicted reactivity
Arabidopsis thaliana, Hibiscus syriacus, Lotus corniculatus, Solanum dulcamara, Solanum lycopersicum,Vigna unguiculata,
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples

~25 µg/well of total protein extracted freshly from N. benthamiana leaf. Sample 3 is a control, no overexpression. Exact buffer components were: Laemmli buffer (62.5mM Tris-HCl (pH 6.8), 10% glycerol, 1%SDS, 0.005% Bromophenol Blue): and denatured with exact buffer components at 70°C for 10 min. Samples were separated on 10 % SDS-PAGE and blotted for 0.5 h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:2 000 in TBS-T ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10 AgriseraBright (mid picogram detection). Exposure time was 5 minutes (LI-COR Odyssey FC Imaging System).
Image courtesy of Dr Yasin Tumtas, Bozkurt Lab, Imperial College London

Target MW: ~77 kDa
Target cellular localisation: Cytoplasmic
~25 µg/well of total protein extracted freshly from N. benthamiana leaf. Sample 3 is a control, no overexpression. Exact buffer components were: Laemmli buffer (62.5mM Tris-HCl (pH 6.8), 10% glycerol, 1%SDS, 0.005% Bromophenol Blue): and denatured with exact buffer components at 70°C for 10 min. Samples were separated on 10 % SDS-PAGE and blotted for 0.5 h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:2 000 in TBS-T ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AS16 ECL-N-10 AgriseraBright (mid picogram detection). Exposure time was 5 minutes (LI-COR Odyssey FC Imaging System).
Image courtesy of Dr Yasin Tumtas, Bozkurt Lab, Imperial College London
Additional information
Background
Background
This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Product citations
Related products: PAL 1-4 | Phenylalanine ammonia-lyase 1-4
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
This product can b...
This product can b...
From 26 €
AS16 4030 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 ...
10 ...
201 €
AS12 1854 Clonality: Polyclonal Host: Rabbit | Reactivity: Arabidopsis thaliana
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items