PARP2 | Poly [ADP-ribose] polymerase 2

AS16 3838 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

Recombinant Arabidopsis thaliana MBP-PARP2protein (AT2G31320)

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1: 1000 (WB)

Expected | apparent MW

72.2 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples Application example

Western blot using anti-PARP2 antibodies

100 µg of total protein from 14 days old Arabidopsis thaliana seedlings extracted with extraction buffer (2% SDS, 50 mM Tris-HCl, pH 8.0, protease inhibitor cocktail (Sigma, P9599) 1:100) and denatured with Laemmli sample loading buffer at 70°C for 10 min were separated on 10 % SDS-PAGE and blotted 2h to PVDF using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% milkTBS-T overnight with agitation at 4°C. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent. Exposure time was 2-5 min.

Courtesy of Dr Julia Vainonen, University of Helsinki, Finland

Additional information

Related products

Collection of antibodies for DNA/RNA metabolism and cell cycle

Plant protein extraction buffer

Secondary antibodies

Background

PARP (EC=2.4.2.30) is a protein involved in the base excision repair pathway (BER) and is catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. PARP is a marker of a single-strand breaks (SSBs), Rybaczek and Kowalewicz-Kulbat (2013).

Alternative names: ADPRT 2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthetase 2.