PARP2 | Poly [ADP-ribose] polymerase 2
AS16 3838 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
|Data sheet||Product citations||Add review|
Recombinant Arabidopsis thaliana MBP-PARP2protein (AT2G31320)
100 µg of total protein from 14 days old Arabidopsis thaliana seedlings extracted with extraction buffer (2% SDS, 50 mM Tris-HCl, pH 8.0, protease inhibitor cocktail (Sigma, P9599) 1:100) and denatured with Laemmli sample loading buffer at 70°C for 10 min were separated on 10 % SDS-PAGE and blotted 2h to PVDF using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% milkTBS-T overnight with agitation at 4°C. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent. Exposure time was 2-5 min.
Courtesy of Dr Julia Vainonen, University of Helsinki, Finland
PARP (EC=22.214.171.124) is a protein involved in the base excision repair pathway (BER) and is catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. PARP is a marker of a single-strand breaks (SSBs), Rybaczek and Kowalewicz-Kulbat (2013).
Alternative names: ADPRT 2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthetase 2.
Related products: PARP2 | Poly [ADP-ribose] polymerase 2
This product can b...