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Peanut protein

AS09 566 | Clonality: Polyclonal | Host: Hen Reactivity: Arachis hypogaea (peanut)

Peanut protein in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 566)

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Product Information

Immunogen

Arachis hypogaea protein extract

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid
Quantity 100 µg
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 2- 5 µg/ml (ELISA), 0.1-1 µg/ml (WB)

Reactivity

Confirmed reactivity Peanut proteins
Predicted reactivity Peanut proteins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using chicken anti-peanut protein antibodies

Thirty (30) µg of total protein extracted freshly from defatted lightly roasted peanut flour with borate buffered saline (BBS) solution (100 mM H3BO4, 25 mM Na2B4O7, 75 mM NaCl, and pH 8.6) for 1 hr with constant stirring at 4 °C. Samples were denatured with NuPAGE™ LDS sample buffer containing 50 mM DTT at a 1:4 (v/v) ratio and incubation at 70°C for 5 min. Samples were separated on Novex™ 10-20% Tricine Protein Gels and blotted 7 minutes to nitrocellulose using iBlot dry transfer system. The blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1,000 for 1h/RT with agitation in TBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at RT with agitation. The blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS10 1489) diluted to 1:25,000 in TBS-T for 1h/RT with agitation. The blot was washed as above and developed for 5 min with AgriseraECLBright. Images of the blots were collected using a CCD imager and Quantity One software (Bio-Rad). Exposure time was 20 seconds.

Additional information

Additional information

Antibodies were purified on immobilized peanut proteins.

Related products

Related products AS15 2848 | Anti-Peanut Agglutinin, mouse monoclonal antibodies
AS16 3977 | Anti-Peanut Ara h1, clone 17, rabbit antibodies

antibodies to food proteins

Plant protein extraction buffer

Secondary antibodies

Background

Background

Peanut (Arachis hypogaea) belongs to the legume family. Dietary substances from peanuts can be a cause of allergi reaction in estimated 0.4-0.6% of population.

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