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Ara h1, clone 17

AS16 3977 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arachis hypogaea

Ara h1, clone 17 in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS16 3977)

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Product Information

Immunogen Recombinant peanut allergen Ara h1, UniProt: P43237, amino acid 26-216.
Host Rabbit
Clonality Polyclonal
Purity Protein G purified serum
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications inDirect ELISA (i-ELISA), Western blot (WB)
Recommended dilution 1 : 320 000 (i-ELISA), 1: 1000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Arachis hypogaea
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

Western blot using anti peanut Ara h1, clone 17 antibodies

Thirty (30) µg of total protein extracted freshly from defatted lightly roasted peanut flour with borate buffered saline (BBS) solution (100 mM H3BO4, 25 mM Na2B4O7, 75 mM NaCl, and pH 8.6) for 1 hr with constant stirring at 4°C. Samples were denatured with NuPAGE™ LDS sample buffer containing 50 mM DTT at a 1:4 (v/v) ratio and incubation at 70°C for 5 min. Samples were separated on Novex™ 10-20% Tricine Protein Gels and blotted 7 minutes to nitrocellulose using iBlot dry transfer system. The blot was blocked with 5% milk in phosphate buffered saline (pH 7.4) with 0.1% tween-20 (PBST) for 1h/RT with agitation. The blot was incubated in the primary antibody at a dilution of 1: 1,000 for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times with 10mL for 5 min in PBST at RT with agitation. The blot was incubated with Donkey anti-Rabbit IRDye 680RD secondary antibody (LI-COR, Nebraska, US) diluted to 1:10,000 in PBST for 1h/RT with agitation. The blot was washed as above, scanned on a LI-COR Odyssey, and images were collected with the 680nM channel.

Additional information

Additional information Antibody solution contains: 50% Glycerol, 0.01M PBS, PH 7.4 and 0.03% Proclin 300.
Antibody cross reactivity to Ara h2 or Ara h3 has not been tested.

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Background

Background Peanut Ara h1 is a major peanut allergen. It shows significant homology with the vicilin seed storage protein found in most higher plants.

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