Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)

AS18 4198-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)

Product Information

Immunogen Pectic polysaccharide (alpha-1,5-arabinan), neoglycoprotein (arabinoheptaose-BSA conjugated),

Host Rat

Clonality Monoclonal

Subclass/isotype IgG

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunoflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides.
In some species this antibody could recognize arabinogalactan-proteins (AGPs).

In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.

Product citations

Selected references Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.

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Clonality:	Monoclonal
Format:	Liquid
Host:	Rat
immunogen:	Pectic polysaccharide (alpha-1,5-arabinan), neoglycoprotein (arabinoheptaose-BSA conjugated),
Purity:	Cell culture supernatant.
Quantity:	1 ml
recommended dilution:	1:10 (ELISA, IF)
storage:	Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Sub class:	IgG
tested applications:	ELISA (ELISA), Immunoflourescence (IF)
additional information:	Contains 0.05% Sodium Azide No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides. In some species this antibody could recognize arabinogalactan-proteins (AGPs). In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.
Confirmed reactivity:	Higher plants, ferns and mosses
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
All references:	Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.