Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)
AS18 4197-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide (beta 1,4-galactan)
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgG
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Contains 0.05% Sodium Azide.
No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan.
No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.Product citations
Selected references
Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.
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