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Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)

AS18 4197-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4197-1ml)
Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)



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Product Information

Immunogen Pectic polysaccharide (beta 1,4-galactan)
Host Rat
Clonality Monoclonal
Subclass/isotype IgG
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide.

No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan.

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Background

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.
additional information: Contains 0.05% Sodium Azide.

No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan.
All references: Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3.
Clonality: Monoclonal
Format: Liquid
Host: Rat
immunogen: Pectic polysaccharide (beta 1,4-galactan)
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution: 1:10 (ELISA, IF)
storage: Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Sub class: IgG
tested applications: ELISA (ELISA), Immunoflourescence (IF)
Confirmed reactivity: Higher plants, ferns and mosses
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background:

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

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