IKP

Heteromannan (monoclonal, clone LM21)

Product no: AS18 4209-1ml
AS18 4209-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
260
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Polysaccharide Heteromannan
    Sub class: IgM
    Host: Rat
    Clonality: Monoclonal
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at +4C (short term) and at -20C (long term).
    Tested applications: ELISA (ELISA), Immuniflourescence (IF)
    Recommended dilution: 1:10 (ELISA, IF)
  • Reactivity
  • Confirmed reactivity: Higher plants, ferns and mosses
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Contains 0.05% Sodium Azide.

    No cross-reactivity with other polymers
    This antibody recognises B-linked mannan polysaccharides of plant cell walls.
    LM21 binds effectively to B-(1-4)-manno-oligosaccharides from DP2 to DP5.
  • Background
  • Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
  • Product Citations
  • Selected references: Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi:0.1111/j.1365-313X.2010.04319.x.
  • Protocols
  • Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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