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Heteromannan (monoclonal, clone LM21)

AS18 4209-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Heteromannan (monoclonal, clone LM21) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4209-1ml)
Heteromannan (monoclonal, clone LM21)



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Product Information

Immunogen Polysaccharide Heteromannan
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide.

No cross-reactivity with other polymers
This antibody recognises B-linked mannan polysaccharides of plant cell walls.
LM21 binds effectively to B-(1-4)-manno-oligosaccharides from DP2 to DP5.

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Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.

Product citations

Selected references Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi:0.1111/j.1365-313X.2010.04319.x.
immunogen: Polysaccharide Heteromannan
Sub class: IgM
Host: Rat
Clonality: Monoclonal
Purity: Cell culture supernatant.
Format: Liquid
Quantity: 1 ml
storage: Store at +4°C (short term) and at -20°C (long term).
Tested applications: ELISA (ELISA), Immuniflourescence (IF)
recommended dilution: 1:10 (ELISA, IF)
Confirmed reactivity: Higher plants, ferns and mosses
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Contains 0.05% Sodium Azide.

No cross-reactivity with other polymers
This antibody recognises B-linked mannan polysaccharides of plant cell walls.
LM21 binds effectively to B-(1-4)-manno-oligosaccharides from DP2 to DP5.
background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
All references: Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi:0.1111/j.1365-313X.2010.04319.x.

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