Pectic polysaccharide, homogalacturonan (monoclonal, clone LM19)
AS18 4191-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide, Homogalacturonan,
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.
Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.
Additional information
Additional information
Contains 0.05% Sodium Azide.
Has no known cross-reactivity with other polymers.
Binds to unesterified homogalacturonan.
The antibody recognizes a range of homogalacturonan samples but binds strongly to un-esterified homogalacturonan.
Has no known cross-reactivity with other polymers.
Binds to unesterified homogalacturonan.
The antibody recognizes a range of homogalacturonan samples but binds strongly to un-esterified homogalacturonan.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.Product citations
Selected references
Marcus et al. (2010). Restricted access of proteins to mannan polysaccharides in intact plant cell walls. Plant J. 2010 Oct;64(2):191-203.doi: 0.1111/j.1365-313X.2010.04319.x.
Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
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