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Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM5)

AS18 4194-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM5) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4194-1ml)
Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM5)



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Pectic polysaccharide, Homogalacturonan domain,
Host Rat
Clonality Monoclonal
Subclass/isotype IgG
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

Has no known cross-reactivity with other polymers.
Binds to paritally methyl esterified homogalacturonan and can also bind to un-esterified homogalacturonan.

Related products

Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. 

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Zhang et al. (2022) Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development, Journal of Plant Physiology, 2022, 153631, ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2022.153631.
Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.

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