Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)

AS18 4195-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7) in the group Antibodies for Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4195-1ml)


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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Pectic polysaccharide, Homogalacturonan.
Host Rat
Clonality Monoclonal
Subclass/isotype IgA
Format Cell Culture Supernatant
Quantity 1 ml
Storage Store at +4°C (short term) and at -20°C (long term).

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)


Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonan.

This antibody is a good marker for pectic homogalacturonan.

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The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.