Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)

Product no: AS18 4195-1ml
AS18 4195-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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  • Product Info
  • Immunogen: Pectic polysaccharide, Homogalacturonan,
    Sub class: IgA
    Host: Rat
    Clonality: Monoclonal
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at +4C (short term) and at -20C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immuniflourescence (IF)
    Recommended dilution: 1:10 (ELISA, IF)
  • Reactivity
  • Confirmed reactivity: Higher plants, ferns and mosses
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Contains 0.05% Sodium Azide

    Has no known cross-reactivity with other polymers.
    Binds to methyl esterified homogalacturonan.
    Does not bind to un-esterified homogalacturonan.

    This antibody is a good marker for pectic homogalacturonan.
  • Background
  • Background:

    The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.  

    Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

  • Product Citations
  • Selected references: Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
    Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.
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From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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AS18 4193-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
246 €
Add to cart
Info
AS18 4194-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
246 €
Add to cart
Info
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