Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)

Name: Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)
SKU: AS18 4195-1ml
Price: 246 EUR
Availability: InStock

AS18 4195-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7) in the group Antibodies Plant/Algal / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4195-1ml)

Pectic polysaccharide, homogalacturonan (monoclonal, clone JIM7)

Product Information

Immunogen Pectic polysaccharide, Homogalacturonan,

Host Rat

Clonality Monoclonal

Subclass/isotype IgA

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immuniflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide

Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonan.

This antibody is a good marker for pectic homogalacturonan.

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6.
Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.

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immunogen:	Pectic polysaccharide, Homogalacturonan,
Sub class:	IgA
Host:	Rat
Clonality:	Monoclonal
Purity:	Cell culture supernatant.
Format:	Liquid
Quantity:	1 ml
storage:	Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immuniflourescence (IF)
recommended dilution:	1:10 (ELISA, IF)
Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Contains 0.05% Sodium Azide Has no known cross-reactivity with other polymers. Binds to methyl esterified homogalacturonan. Does not bind to un-esterified homogalacturonan. This antibody is a good marker for pectic homogalacturonan.
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
All references:	Clausen et al. (2003). Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 003 Aug 12;338(17):1797-800.doi: 10.1016/s0008-6215(03)00272-6. Knox et al. (1990). Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta. 1990 Jul;181(4):512-21.doi: 0.1007/BF00193004.